ir. Z.A. (Michal) Otterloo

ir. Z.A. (Michal) Otterloo

Associate Professor
ir. Z.A. (Michal) Otterloo
  • CDL Research
  • Departement of Experimental Cardiology

Research Programs



In my research, I focus on uncovering the biological mechanisms of atherosclerosis and heart failure. I utilize bulk and single-cell transcriptomics, epigenetics, bioinformatics and combine those with clinical data sets and in vitro experiments. 

My research utilizes the largest biobank of well-characterized atherosclerotic plaques with more than 4000 included patients – the Athero-Express study. We generate various types of complex “-omics” datasets, including genotyping, transcriptomics (bulk, single-cell and spatially resolved), DNA methylation (bulk and single-cell) and proteomics. These datasets are integrated with histological and clinical data to unravel mechanisms in plaque pathogenesis and allow us to identify specific processes that lead to the severe manifestation of atherosclerotic disease like stroke and myocardial infarction. Next to computational tools, we use primary in vitro model systems derived from vascular and plaque cells and molecular biology techniques for validation and mechanistic studies.

Research Output (102)

The Applications of Single-Cell RNA Sequencing in Atherosclerotic Disease

Slenders Lotte, Tessels Daniëlle E, van der Laan Sander W, Pasterkamp Gerard, Mokry Michal 8 Feb 2022, In: Frontiers in cardiovascular medicine. 9 , p. 826103

Intersecting single-cell transcriptomics and genome-wide association studies identifies crucial cell populations and candidate genes for atherosclerosis

Slenders Lotte, Landsmeer Lennart P L, Cui Kai, Depuydt Marie A C, Verwer Maarten, Mekke Joost, Timmerman Nathalie, van den Dungen Noortje A M, Kuiper Johan, de Winther Menno P J, Prange Koen H M, Ma Wei Feng, Miller Clint L, Aherrahrou Redouane, Civelek Mete, de Borst Gert J, de Kleijn Dominique P V, Asselbergs Folkert W, den Ruijter Hester M, Boltjes Arjan, Pasterkamp Gerard, van der Laan Sander W, Mokry Michal Jan 2022, In: European heart journal open. 2

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Pei Jiayi, van den Dungen Noortje A M, Asselbergs Folkert W, Mokry Michal, Harakalova Magdalena 2022, 2458 , p. 97-111 15 p.

CCN2 Aggravates the Immediate Oxidative Stress–DNA Damage Response following Renal Ischemia–Reperfusion Injury

Valentijn Floris, Goldschmeding Roel, Marquez Exposito Laura, Broekhuizen R., Nguyen Tri Q., Knoppert Sebastiaan, Falke LL, Kester Lennart, Mokry Michal, Ruiz-Ortega Marta, Rodrigues-Diez Raúl R. 20 Dec 2021, In: Antioxidants. 10 , p. 1-16

The changing landscape of the vulnerable plaque:a call for fine-tuning of preclinical models

Buono Michele F, Slenders Lotte, Wesseling Marian, Hartman Robin J G, Monaco Claudia, den Ruijter Hester M, Pasterkamp Gerard, Mokry Michal Dec 2021, In: Vascular Pharmacology. 141 , p. 1-10

Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations

Pei J, Schuldt M, Nagyova E, Gu Z, El Bouhaddani S, Yiangou L, Jansen M, Calis J J A, Dorsch L M, Blok C Snijders, van den Dungen N A M, Lansu N, Boukens B J, Efimov I R, Michels M, Verhaar M C, de Weger R, Vink A, van Steenbeek F G, Baas A F, Davis R P, Uh H W, Kuster D W D, Cheng C, Mokry M, van der Velden J, Asselbergs F W, Harakalova M Dec 2021, In: Clinical Epigenetics. 13 , p. 1-20

Conserved human effector Treg cell transcriptomic and epigenetic signature in arthritic joint inflammation

Mijnheer Gerdien, Lutter Lisanne, Mokry Michal, van der Wal Marlot, Scholman Rianne, Fleskens Veerle, Pandit Aridaman, Tao Weiyang, Wekking Mark, Vervoort Stephin, Roberts Ceri, Petrelli Alessandra, Peeters Janneke G C, Knijff Marthe, de Roock Sytze, Vastert Sebastiaan, Taams Leonie S, van Loosdregt Jorg, van Wijk Femke Dec 2021, In: Nature Communications. 12 , p. 1-16

Enhanced single-cell RNA-seq workflow reveals coronary artery disease cellular cross-talk and candidate drug targets

Ma Wei Feng, Hodonsky Chani J, Turner Adam W, Wong Doris, Song Yipei, Mosquera Jose Verdezoto, Ligay Alexandra V, Slenders Lotte, Gancayco Christina, Pan Huize, Barrientos Nelson B, Mai David, Alencar Gabriel F, Owsiany Katherine, Owens Gary K, Reilly Muredach P, Li Mingyao, Pasterkamp Gerard, Mokry Michal, van der Laan Sander W, Khomtchouk Bohdan B, Miller Clint L 26 Nov 2021, In: Atherosclerosis. 340 , p. 12-22 11 p.

Sex-dependent gene co-expression in the human body

Hartman Robin J G, Mokry Michal, Pasterkamp Gerard, den Ruijter Hester M 21 Sep 2021, In: Scientific Reports. 11

Activin-A Induces Early Differential Gene Expression Exclusively in Periodontal Ligament Fibroblasts from Fibrodysplasia Ossificans Progressiva Patients

Schoenmaker Ton, Mokry Michal, Micha Dimitra, Netelenbos Coen, Bravenboer Nathalie, Gilijamse Marjolijn, Eekhoff E Marelise W, de Vries Teun J 1 Jun 2021, In: Biomedicines. 9 , p. 1-16

All research output

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